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Biomarkers can be used to comprehensively monitor the physiological status of ASD patients during diagnosis, intervention, and treatment, which can aid in understanding the condition, judging the treatment strategy, and monitoring efficacy and prognosis ( Currently, mass spectrometry-based targeted proteomic or metabolomic approaches can effectively monitor multiple disease markers simultaneously (274) and utilized (275)

*Cytokines: Signaling molecules that mediate and regulate immunity, inflammation and hematopoiesis *TNF: Tumor necrosis factor

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